Continuous production of avian sarcoma virus B77 by rat tumour cells in tissue culture

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Abstract
The rat tumour RBI, previously induced by injection of fowl sarcoma B77 tissue into newborn rats, was adapted to long‐term growth in tissue culture. Two cell lines, 16B177 and 17B177, were derived from the tumour. Both cell lines were capable of continuous production and release of infectious B77 virus over a period of more than two years' in vitro cultivation. The virus present in cell‐free tissue culture media induced characteristic sarcomas upon inoculation into young chicks.Preliminary chromosomal analysis of the uncloned population of 17BI77 cells confirmed their rat origin and revealed that the hypotriploid stem‐line predominates, being represented by 59 chromosomes.