Experimental Galactose Toxicity: Effects on Synaptosomal Phosphatidylinositol Metabolism

Publisher Website
Google Scholar
Abstract
Experimental galactose toxicity was induced by weaning rats onto an isocaloric 40% galactose diet. Synaptosomes were prepared from cerebra of rats at 2‐9 weeks post‐weaning and incubated with [33P]Pi and myo‐[2‐3H]inositol in the presence or absence of 0.2 mM‐acetylcholine. The acetylcholine‐stimulated [33P]Pi labeling of phosphatidylinositol and the changes in amounts of phosphatidylinositol were similar in the normal and galactose‐toxic rats; however, acetylcholine‐stimulated myo‐[2‐3H]inositol labeling of phosphatidylinositol was markedly decreased in the galactose‐toxic rats. The impairment of acetylcholine‐stimulated myo‐[2‐3H]inositol incorporation into phosphatidylinositol observed after 2 weeks on the diet did not vary after more prolonged exposure to galactose.