Role of His243 in the phosphatase activity of EnvZ in Escherichia coli
- 1 February 1997
- journal article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 179 (4) , 1413-1416
- https://doi.org/10.1128/jb.179.4.1413-1416.1997
Abstract
EnvZ undergoes autophosphorylation at His243 and subsequently transfers the phosphate group to OmpR. EnvZ also possesses an OmpR-phosphate phosphatase activity. We examined the role of His243 in the phosphatase function by replacing His with either Val, Tyr, Ser, Asp, or Asn. EnvZH243V and EnvZH243Y were both shown to possess phosphatase activity in vitro. In addition, the mutant proteins were able to reduce the high level of OmpR-phosphate present in the envZ473 strain. These results indicate that His243 of EnvZ is not essential for stimulating the dephosphorylation of OmpR-phosphate.Keywords
This publication has 23 references indexed in Scilit:
- Reverse Phosphotransfer from OmpR to EnvZ in a Kinase−/Phosphatase+ Mutant of EnvZ (EnvZ•N347D), a Bifunctional Signal Transducer of Escherichia coliJournal of Biological Chemistry, 1996
- Signal transduction by the EnvZ-OmpR phosphotransfer system in bacteriaResearch in Microbiology, 1994
- Identification of a phosphorylation site and functional analysis of conserved aspartic acid residues of OmpR, a transcriptional activator for ompF and ompC in Escherichia coliMolecular Microbiology, 1993
- Requirement of Both Kinase and Phosphatase Activities of an Escherichia coli Receptor (Taz1) for Ligand-dependent Signal TransductionJournal of Molecular Biology, 1993
- Intermolecular complementation between two defective mutant signal-transducing receptors of Escherichia coli.Proceedings of the National Academy of Sciences, 1991
- EnvZ controls the concentration of phosphorylated OmpR to mediate osmoregulation of the porin genesJournal of Molecular Biology, 1991
- Signal transduction and gene regulation through the phosphorylation of two regulatory components: the molecular basis for the osmotic regulation of the porin genesMolecular Microbiology, 1990
- Phosphorylation and dephosphorylation of a bacterial transcriptional activator by a transmembrane receptor.Genes & Development, 1989
- Phosphorylation of OmpR by the osmosensor EnvZ modulates expression of the ompF and ompC genes in Escherichia coli.Proceedings of the National Academy of Sciences, 1989
- Phosphorylation of nitrogen regulator I (NRI) of Escherichia coli.Proceedings of the National Academy of Sciences, 1988