Inhibition of DNA synthesis in cultures of 3T3 cells by isolated surface membranes.
- 1 June 1977
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 74 (6) , 2251-2255
- https://doi.org/10.1073/pnas.74.6.2251
Abstract
When added to a sparse culture of 3T3 [mouse fibroblasts] cells, a surface membrane-enriched fraction from 3T3 cells inhibited rate of DNA synthesis in a time- and concentration-dependent manner. Membrane preparation had no effect on rate of DNA synthesis of SV 40-transformed 3T3 cells. A similar membrane preparation from transformed cells had a lesser inhibitory effect on 3T3 cells and no effect on transformed cells. The inhibition by membranes were reversible. When added to growing 3T3 cells, 3T3 surface membranes may mimic the effect of increasing cell density on DNA synthesis.This publication has 20 references indexed in Scilit:
- Tumorigenicity of virus-transformed cells in nude mice is correlated specifically with anchorage independent growth in vitro.Proceedings of the National Academy of Sciences, 1975
- Growth inhibition of animal cells by succinylated concanavalin ANature, 1975
- Shaking 3T3 cells: Further studies on diffusion boundary effectsCell, 1974
- A Restriction Point for Control of Normal Animal Cell ProliferationProceedings of the National Academy of Sciences, 1974
- Plasma Membranes from Cultured Muscle Cells: Isolation Procedure and Separation of Putative Plasma-Membrane Marker EnzymesProceedings of the National Academy of Sciences, 1973
- Binding of [3H]Concanavalin A to Normal and Transformed CellsJournal of Biological Chemistry, 1973
- PLASMA MEMBRANES OF MAMMALIAN CELLSThe Journal of cell biology, 1973
- Topoinhibition and Serum Requirement of Transformed and Untransformed CellsNature, 1970
- GROWTH CHARACTERISTICS OF VIRUS-TRANSFORMED CELLSThe Journal of Experimental Medicine, 1970
- THE DIFFERENCE IN CONTACT INHIBITION OF CELL REPLICATION BETWEEN NORMAL CELLS AND CELLS TRANSFORMED BY DIFFERENT CARCINOGENSProceedings of the National Academy of Sciences, 1966