Short communication: PCR‐based detection of the polymorphism at codon 136 in the ovine prion protein gene
- 1 May 2000
- journal article
- other
- Published by Taylor & Francis in Animal Biotechnology
- Vol. 11 (1) , 69-73
- https://doi.org/10.1080/10495390009525949
Abstract
In many breeds of sheep, a polymorphism at codon 136 of the prion protein gene has been shown to be strongly associated with the risk of developing scrapie. A single‐step procedure for detecting this allelic variation is described here. When performed on a series of animals, the test was in complete agreement with their genotypes as had been previously determined by sequencing. The test is potentially easier and quicker to perform than any of the variety of methods that are currently used for this purpose.Keywords
This publication has 12 references indexed in Scilit:
- Association between natural scrapie and PrP genotype in a flock of Suffolk sheep in ScotlandVeterinary Record, 1997
- Allelic frequencies of an ovine scrapie susceptibility geneAnimal Biotechnology, 1996
- Identification of five allelic variants of the sheep PrP gene and their association with natural scrapieJournal of General Virology, 1995
- PrP Genotype and Agent Effects in Scrapie: Change In Allelic Interaction With Different Isolates of Agent in Sheep, a Natural Host of ScrapieJournal of General Virology, 1994
- The association of a codon 136 PrP gene variant with the occurrence of natural scrapieArchiv für die gesamte Virusforschung, 1994
- Swaledale sheep affected by natural scrapie differ significantly in PrP genotype frequencies from healthy sheep and those selected for reduced incidence of scrapieJournal of General Virology, 1993
- PrP Polymorphisms Associated with Natural Scrapie Discovered by Denaturing Gradient Gel ElectrophoresisGenomics, 1993
- Different scrapie-associated fibril proteins (PrP) are encoded by lines of sheep selected for different alleles of the Sip geneJournal of General Virology, 1991
- Restriction fragment length polymorphisms of the scrapie-associated fibril protein (PrP) gene and their association with susceptibility to natural scrapie in British sheepJournal of General Virology, 1991
- Two alleles of a neural protein gene linked to scrapie in sheep.Proceedings of the National Academy of Sciences, 1990