Nitric oxide stimulates the ADP-ribosylation of a 41-kDa cytosolic protein in Dictyostelium discoideum.
- 1 July 1992
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 89 (13) , 5902-5906
- https://doi.org/10.1073/pnas.89.13.5902
Abstract
Nitric oxide-releasing compounds were shown to activate an ADP-ribosyltransferase activity in the cytosol of Dictyostelium discoideum. The enzyme ADP-ribosylated a cytosolic protein of approximately 41 kDa, p41. Neither cGMP nor GTP and its analogues affected this ADP-ribosylation. p41 differs from other substrates ADP-ribosylated by cholera, pertussis, or diphtheria toxins. Treatment of ADP-ribosylated p41 with snake venom phosphodiesterase released adenosine 5'-monophosphate, indicating a mono-ADP-ribose-protein linkage. This linkage was stable to neutral hydroxylamine but was sensitive to mercury ions and iodomethane, suggesting an attachment to a cysteine residue. Treatment of intact cells with nitric oxide-releasing compounds appeared to stimulate the ADP-ribosylation of p41 and this modification was reversible.Keywords
This publication has 44 references indexed in Scilit:
- Reversible ADP‐ribosylation of the 78 kDa glucose‐regulated proteinFEBS Letters, 1990
- G Proteins in Signal TransductionAnnual Review of Pharmacology and Toxicology, 1990
- Stimulation of mono (ADP‐ribosyl)ation by reduced extracellular calcium levels in human fibroblastsJournal of Cellular Physiology, 1988
- Adp‐Ribosylation of Guanyl Nucleotide‐Binding Regulatory Proteins by Bacterial ToxinsPublished by Wiley ,1988
- Pertussis and cholera toxin ADP-ribosylation in dictyostelium discoideum membranesBiochemical and Biophysical Research Communications, 1987
- Regulation of Dictyostelium discoideum adenylate cyclase by manganese and adenosine analogsBiochimica et Biophysica Acta (BBA) - Molecular Cell Research, 1987
- On the nature of cellular ADP-ribosyltransferase from rat liver specific for elongation factor 2Biochemical and Biophysical Research Communications, 1986
- Occurrence of the adenylate cyclase “G Protein” in membranes of DictyosteliumdiscoideumBiochemical and Biophysical Research Communications, 1981
- The obligatory role of endothelial cells in the relaxation of arterial smooth muscle by acetylcholineNature, 1980
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970