Influence of Amphiphilic Mobile Phase Additives Upon the Direct Liquid Chromatographic Optical Resolution by Means of BSA-Based Chiral Sorbents

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Abstract
The effect of amphiphilic retention modifiers in liquid chromatography on BSA as a chiral stationary phase has been investigated. In all cases studied, capacity ratios are decreased or essentially unchanged. Decreasing capacity ratios affected the separation factors quite differently, depending on the nature of the analyte. In some cases increased α-values were found. Anionic modifiers (C6 - C10 alkanoic acids) cause the largest reduction of the capacity ratios in a series of carboxylic acid analytes and are also most tightly bound to the stationary phase. The effects of hexylamine, hexanol and hexanoic acid upon the retention and resolution of an uncharged analyte (oxazepam) were very similar and not very large, indicating very little influence on the chiral binding site. The large effects on anionic analytes were not in good agreement with an ion-pair distribution model.