Polarity of gene conversion and post-meiotic segregation at the buff locus in Sordaria brevicollis

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Abstract
SUMMARY: The flanking markers of wild-type recombinant spores originating from crosses of spore colour mutants in Sordaria brevicollis were analysed. Recombinant asci were of two main types – either with one or with two wild-type spores. In most crosses the behaviour of flanking markers was significantly different for these two types of recombinant asci. The main differences were in the polarity of gene conversion (as inferred from parental outside marker combinations) and in the frequency of recombinant outside markers. These differences were interpreted in terms of hybrid DNA models of recombination and correction of heteroduplex DNA.