IMMUNIZATION AGAINST ANAPLASMOSIS AND BABESIOSIS .2. EVALUATION OF CRYO-PRESERVED VACCINES USING DIFFERENT DOSES AND ROUTES OF INOCULATION

  • 1 January 1978
    • journal article
    • research article
    • Vol. 29  (2) , 210-214
Abstract
Anaplasma marginale, Babesia argentina and B. bigemina infected blood used as vaccines for immunization trials in Valle de Cauca [Colombia], were preserved with 4 M dimethyl-sulfoxide (DMSO) and stored in liquid N (-196.degree. C). The effectivity of the vaccines was determined in 87 healthy calves utilizing serial 10-fold dilutions. The effects of dose, inoculation routes, time and temperature were determined. The minimum infective dose for A. marginale was 10-3 (2 .times. 106) when 2 ml of vaccine were given i.v. The same dose when given s.c. was not infective. The 10-2 dilution (2 .times. 107) was infective when given through both routes, however, the incubation periods were statistically different. The average incubation period using 2 ml s.c. was 30 days, but when the dose was increased to 5 ml and given s.c. the average incubation period decreased to 22 days. The minimum infective doses for B. bigemina and B. argentina were 10-1 dilutions (4 .times. 10-7) and 10-2 (4 .times. 166), respectively, when 2 ml of vaccines were injected i.v.Infectivity was also recorded when Babesia spp. vaccines were injected s.c. at dosages of 5 ml of dilution 10-1 (1 .times. 10-8).