Interleukin‐10 prevents the generation of dendritic cells from human peripheral blood mononuclear cells cultured with interleukin‐4 and granulocyte/ macrophage‐colony‐stimulating factor

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Abstract
We evaluated the effects of interleukin (IL)‐10 on the differentiation of dendritic cells (DC) obtained by culturing plastic‐adherent peripheral blood mononuclear cells for 7 days in presence of granulocyte/macrophage‐colony‐stimulating factor (GM‐CSF)+IL‐4. The addition of IL‐10 at the initiation of culture resulted in the generation of macrophage‐like cells with expressing high levels of CD14 and decreased levels of CD1a and CD1c. Furthermore, cells generated in presence of IL‐10 secreted lower levels of IL‐12, but higher levels of IL‐8 compared with DC generated in absence of IL‐10, both spontaneously and after CD40 engagement. Finally, cells generated in presence of IL‐10 were less efficient than DC in stimulating the production of IL‐2, interferon‐γ, and IL‐4 by allogeneic T cells. We conclude that IL‐10 prevents the generation of DC induced by GM‐CSF+IL‐4 and favors the development of macrophages with a lower T cell stimulatory potential, but secreting higher levels of IL‐8 than DC.

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