Effects of photolysable 2-azido analogues of adenosine, AMP and ADP on human platelets

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Abstract
The aggregating effect of ADP on human platelets and the inhibiting effect of adenosine are apparently mediated by different receptors on the cells’ outer membranes. Photolysable azido-analogues of adenosine, AMP and ADP have been prepared so that the receptors for them can be labelled. 2-Azidoadenosine inhibited platelet aggregation by ADP more than adenosine itself and increased platelet cAMP as effectively as did adenosine or 2-chloroadenosine. 2-Azido-AMP inhibited aggregation much more effectively than AMP itself or than 2-chloro-AMP. 2-Azido-ADP was about five times more potent than ADP in causing aggregation. All these 2-azido derivatives were photolysable by irradiation at 365 nm which does not affect platelet functions.