Absence of polyadenylate segment in globin messenger RNA accelerates its degradation in Xenopus oocytes.
- 1 August 1975
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 72 (8) , 3065-3067
- https://doi.org/10.1073/pnas.72.8.3065
Abstract
Rabbit globin poly(A)-free and native mRNA preparations were microinjected into Xenopus oocytes. The amount of globin message remaining after incubation of injected oocytes was determined by molecular hybridization with a radioactive complementary DNA probe, synthesized by copying globin mRNA with purified RNA-dependent DNA polymerase (reverse transcriptase) from avian myeloblastosis virus. The results show that 56 hr after injection about 85% of the poly(A)-free mRNA molecules are degraded, while native poly(A)-containing mRNA chains are almost completely preserved during the same period of time.Keywords
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