ONE-STAGE STIMULATION OF HUMAN LYMPHOCYTE-T COLONY-FORMING-UNITS (TL-CFU) IN A MICRO AGAR CULTURE IN GLASS-CAPILLARIES

  • 1 January 1979
    • journal article
    • research article
    • Vol. 38  (2) , 393-399
Abstract
A 1-stage micro-culture assay in glass capillary tubes were developed for colony growth of human T [thymus-derived] lymphocytes. Colony growth by this method did not depend on addition of growth factors, human serum and erythrocytes. Advantages of this method are low material consumption and the high numbers of cultures prepared and evaluated within a relatively short time period. The 1-stage method requires a higher number of seeded cells and a lower agar concentration than the 2-stage method. Cell interactions may be required for human T lymphocyte colony formation.