Abstract
In salivary glands of Chironomus tentans larvae, RNA and protein synthesis were inhibited with actinomycin D and cycloheximide, respectively. If ecdysone was injected at the beginning of the temporal inhibition of RNA synthesis, the first induced puffs appeared only after resumption of RNA synthesis, and the schedule of sequential puffing started from here instead of from the injection. Inhibition of protein synthesis did not interfere with induction of the early responding puffs. The sequence, however, stopped after these puffs had appeared.