Glucose dehydrogenase: a new oxidising enzyme from animal tissues

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Abstract
This enzyme was obtained by saturating the water extract of acetone-liver with (NH4)2SO4. It has been extracted from the liver of the ox, sheep, cat and dog, and is obtainable in a clear, cell-free solution which can be filtered through a porcelain candle or through kieselguhr. It brings about the oxidation of glucose in presence of methylene blue as H acceptor. This anaerobic oxidation of glucose is not inhibited by KCN, NaF, toluene or iodoacetic acid. Glucose is not oxidised in the presence of O, an O activator apparently being necessary. The system glucose and glucose dehydrogenase has been combined with Keilin''s cytochrome-indophenol oxidase system in presence of which the aerobic oxidation of glucose readily occurs, cytochrome acting as a carrier between active O from the oxidase and active H from glucose plus its dehydrogenase. The oxidation product, of glucose is gluconic acid.

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