Fluorescence In Situ Hybridization in Paraffin Tissue Sections

1 January 2002

journal article
research article
Published by Springer Nature in Molecular Biotechnology

Vol. 21 (3) , 217-220
https://doi.org/10.1385/mb:21:3:217

Abstract

Fluorescence in situ hybridization has found wide application in the enumeration of gene and chromosome copy number both in isolated cells and in tissue sections. However, the technique has been less widely used than would be expected in formalin-fixed paraffin processed (archival) tissue. This article describes a method for assessing archival tissue sections, following pretreatment, before applying DNA probes, that gives consistent, reliable results.

Keywords

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