FURTHER STUDIES ON RNA AND PROTEIN BIOSYNTHESIS IN THE OVARIECTOMIZED MOUSE UTERUS

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Abstract
RNA was isolated from the ovarian and uterine tissues of calves by the cold phenol procedure. 10 OD of uterine-RNA, ovarian-RNA or the equivalent volume of normal saline was injected into the uterine lumen of ovariectomized mice. Alkaline phosphatase and [beta]-glucuronidase levels in the uterus were studied 3 days following the intra-uterine injections. Results indicate that both U-RNA and O-RNA were able to induce protein (enzyme) production in the atrophied mouse uterus. Although U-RNA caused higher [beta]-glu-curonidase content of the uterus than O-RNA, the physiological state of the tissue used to supply the RNA did not have any effect. Ribo-nuclease and KOH digestion of the RNA extract abolishes its action. Actinomycin D given simultaneously with RNA represses the latter''s action. The possibility of hormonal contamination in the RNA extraction is discussed and should be ruled out after ether washing.