Pinpointing phosphotyrosine‐dependent interactions downstream of the collagen receptor DDR1
- 1 December 2005
- journal article
- Published by Wiley in FEBS Letters
- Vol. 580 (1) , 15-22
- https://doi.org/10.1016/j.febslet.2005.11.035
Abstract
Activation of the receptor tyrosine kinase DDR1 by collagen results in robust and sustained phosphorylation, however little is known about its downstream mediators.Using phosphopeptide mapping and site-directed mutagenesis, we here identified multiple tyrosine phosphorylation sites within DDR1. We found that Nck2 and Shp-2, two SH2 domain-containing proteins, bind to DDR1 in a collagen-dependent manner. The binding site of Shp-2 was mapped to tyrosine-740 of DDR1 within an ITIM-consensus sequence. Lastly, ablation of DDR1 in the mouse mammary gland resulted in delocalized expression of Nck2, suggesting that defects observed during alveologenesis are caused by the lack of the DDR1–Nck2 interactionKeywords
This publication has 38 references indexed in Scilit:
- Structure of an Ultraweak Protein-Protein Complex and Its Crucial Role in Regulation of Cell Morphology and MotilityMolecular Cell, 2005
- The Murine Nck SH2/SH3 Adaptors Are Important for the Development of Mesoderm-Derived Embryonic Structures and for Regulating the Cellular Actin NetworkMolecular and Cellular Biology, 2003
- Interaction of discoidin domain receptor 1 isoform b (DDR1b) with collagen activates p38 mitogen‐activated protein kinase and promotes differentiation of macrophagesThe FASEB Journal, 2003
- Brain-derived neurotrophic factor promotes interaction of the Nck2 adaptor protein with the TrkB tyrosine kinase receptorBiochemical and Biophysical Research Communications, 2002
- Discoidin domain receptor 1 isoform‐a (DDRla) promotes migration of leukocytes in three‐dimensional collagen latticesThe FASEB Journal, 2001
- Nck/Dock: an adapter between cell surface receptors and the actin cytoskeletonOncogene, 2001
- Identification of two novel, kinase‐deficient variants of discoidin domain receptor 1: differential expression in human colon cancer cell linesThe FASEB Journal, 2001
- The discoidin domain receptor tyrosine kinase DDR1 in arterial wound repairJournal of Clinical Investigation, 2001
- Roles for the protein tyrosine phosphatase SHP-2 in cytoskeletal organization, cell adhesion and cell migration revealed by overexpression of a dominant negative mutantOncogene, 2000
- Protein-tyrosine Phosphatase 1D Modulates Its Own State of Tyrosine PhosphorylationJournal of Biological Chemistry, 1995