Recovery of Growth Regulator from Plants Treated with 2,4-Dichlorophen-oxyacetic Acid

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Abstract
A 0.0025 ml. drop of 95% ethanol containing 5[mu]g. of 2,4-D and 25ug. of Tween-20 was placed near the base of each of the 2 primary leaves of 141 eight-day-old Black Valentine bean seedlings. After 24 hrs. the primary leaves were removed and the remainder of the above ground portions were dried, ground, and 2-g. aliquots were extracted by refluxing with 80% alcohol followed by purification of the extract. The 2,4-D content of the resulting soln. was detd. by the leaf repression biological assay and expressed in terms of the amt. of 2,4-D that would produce the same repression as the plant extract. Of the total amt. of 2,4-D applied to the leaves, 44% was recovered from the stalks, indicating considerable translocation of this compound in the plant.