PLATELET FACTOR-VIII-RELATED ANTIGEN - STUDIES INVIVO AFTER TRANSFUSION IN PATIENTS WITH VON WILLEBRAND DISEASE

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  • 1 January 1978
    • journal article
    • research article
    • Vol. 51  (4) , 751-761
Abstract
Four unrelated patients with a severe form of von Willebrand disease showed no detectable factor VIII-related antigen (VIIIR:Ag) in their plasma or their platelets. They received cryoprecipitate infusions, 3 patients in a single injection each 1 every day for 9 days before and after surgery. Platelet VIIR:Ag was studied at different times during and after transfusion using electroimmunoassay of platelet extracts and EM of the platelets incubated with anti-VIIIR:Ag antibodies coupled to peroxidase. No VIIIR-Ag was detected in or around the patients'' platelets, although this antigen was detected in the circulating blood. It was suggested that there was no VIIIR:Ag uptake from the plasma by the platelets and that platelet VIIIR:Ag came from megakaryocytes.