Separation of pepsinogen II and pepsinogen III from human urine

Abstract
Human urine has been fractionated on diethylaminoethyl cellulose to yield two pepsinogens. They have been called pepsinogen II and pepsinogen III on the basis of chromatographic behavior identical to that of fundic mucosal pepsinogens II and III. Pepsinogen I has been found absent from 23 urine specimens of 12 individuals. Results suggest a gross qualitative and quantitative correlation between pepsinogen II-pepsinogen III ratio (in terms of total proteolytic activity) in urine and gastric mucosa.

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