Synthesis of ITP and ATP and Regeneration of 2,3‐DPG in Human Erythrocytes Incubated with Adenosine, Pyruvate, and Inorganic Phosphate

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Abstract
In the stored, 2,3-DPG [diphosphoglycerate] depleted human erythrocytes incubated for 4 h in a medium containing adenosine (10 mM), pyruvate (10 mM) and inorganic phosphate (50 mM) the regeneration of 2,3-DPG reached the value of 5 times higher than the physiological concentration and the ATP synthesis exceeded 4 times the physiological level. These erythrocytes are able to synthesize hypoxanthine nucleotides, namely IMP to 58 and ITP to 61 .mu.mol/100 ml of erythrocytes.