Adhesion Forces Between Individual Ligand-Receptor Pairs
- 15 April 1994
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 264 (5157) , 415-417
- https://doi.org/10.1126/science.8153628
Abstract
The adhesion force between the tip of an atomic force microscope cantilever derivatized with avidin and agarose beads functionalized with biotin, desthiobiotin, or iminobiotin was measured. Under conditions that allowed only a limited number of molecular pairs to interact, the force required to separate tip and bead was found to be quantized in integer multiples of 160 +/- 20 piconewtons for biotin and 85 +/- 15 piconewtons for iminobiotin. The measured force quanta are interpreted as the unbinding forces of individual molecular pairs.Keywords
This publication has 17 references indexed in Scilit:
- Sensing Discrete Streptavidin-Biotin Interactions with Atomic Force MicroscopyLangmuir, 1994
- Direct observation of kinesin stepping by optical trapping interferometryNature, 1993
- Force of Single Kinesin Molecules Measured with Optical TweezersScience, 1993
- Direct Mechanical Measurements of the Elasticity of Single DNA Molecules by Using Magnetic BeadsScience, 1992
- From Molecules to Cells: Imaging Soft Samples with the Atomic Force MicroscopeScience, 1992
- Synthetic lipid-anchored receptors based on the binding site of a monoclonal antibodyBiochimica et Biophysica Acta (BBA) - Biomembranes, 1992
- Conformational Substates in ProteinsAnnual Review of Biophysics, 1988
- Optical trapping and manipulation of single cells using infrared laser beamsNature, 1987
- Atomic Force MicroscopePhysical Review Letters, 1986
- Binomial smoothing filter: A way to avoid some pitfalls of least-squares polynomial smoothingReview of Scientific Instruments, 1983