Nutritional Requirements for Axenic Growth of Some Haustorial Mycoparasites

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Abstract
A study of the nutritional conditions required for axenic growth of five biotrophic haustorial mycoparasites, with emphasis on Tieghemiomyces parasiticus, was made on a basal casein hydrolysate medium. Four species failed to utilize glucose or other sugars and sugar utilization by the fifth one is doubtful. Glycerol was utilized readily by all species. Casein hydrolysate was not utilized as a carbon source. Four species were totally deficient for thiamine. T. parasiticus was favored by unusually high concentrations of thiamine and also was highly deficient for biotin. T. parasiticus and Dimargaris verticillata grew more rapidly on media with high concentrations of casein hydrolysate in the presence of glycerol but no growth of either parasite occurred on media with single amino acids. It appears that specific requirements for carbon sources, nitrogen sources, and vitamins may be common among the biotrophic mycoparasites.