RECOMBINANT GAMMA-INTERFERON AND LIPOPOLYSACCHARIDE ENHANCE 1,25-DIHYDROXYVITAMIN-D3-INDUCED CELL-DIFFERENTIATION IN HUMAN PROMYELOCYTIC LEUKEMIA (HL-60) CELLS

  • 1 January 1985
    • journal article
    • research article
    • Vol. 76  (7) , 596-602
Abstract
The induction of cell differentiation by a combination of 1,25-dihydroxyvitamin D3 [1,25-(OH)2D3], recombinant gamma-interferon (rec .gamma.-IFN) and a lipopolysaccharide from E. coli (LPS) was studied in a clonal population (clone-9) of human promyelocytic HL-60 leukemia cells in vitro. Treatment of clone-9 cells with 10-9-10-9-10-7 M 1,25-(OH)2D3 yielded a macrophage cell differentiation. The addition of 10 or 100 U/ml of .gamma.-IFN and 2 or 10 .mu.g/ml LPS caused a further increase in expression of the different differentiation markers. The most pronounced effects involved increases in cell attachment to the surface of tissue-culture Petri dishes and in lysozyme, nonspecific esterase and cytolytic activities. The combined treatment with 1,25-(OH)2D3, rec .gamma.-IFN and LPS also caused an increase in the percent of multinucleated giant cells. These results indicate the effectiveness of combining different agents in inducing cell differentiation in HL-60 cells. A similar approach may be useful in controlling myeloid leukemias in vitro.