Inhibitory effect of chlorophyll on the genotoxicity of 3-amino-1-methyl-5H-pyrido[4,3-b indole (Trp-P-2)
- 1 January 1989
- journal article
- research article
- Published by Oxford University Press (OUP) in Carcinogenesis: Integrative Cancer Research
- Vol. 10 (1) , 145-149
- https://doi.org/10.1093/carcin/10.1.145
Abstract
Genotoxicity of 3-amino-1-methyl-5H-pyrido [4,3-b indole(Trp-P-2) on Drosophila was suppressed by chlorophyll. Using the wing hair spot test, we found that the formation of mutant hairs in adult files as a result of feeding them with Trp-P-2 in their larval stage was efficiently inhibited by coadininistration of chlorophyll. The decrease in the spot frequencies was dependent on the dose of chlorophyll, and at the highest dose used, where the ratio in weight of Trp-P-2 to chlorophyll was 1:80, a complete prevention of the small single-spot formation was observed. A similar inhibitory effect was detected for chlorophyllin, the chromophore of chlorophyll. In the studies to investigate the mechanism of inhibition, we observed that the mutagenicity of 3-hydroxyamino-1-methyl-5H-[4,3-bpyrido [Trp-P-2(NHOH)], the metabolically activated form of Trp-P-2, in Salmonella typhimurium TA98 was suppressed effectively with chlorophyll and chlorophyllin. We also found that chlorophyll and chlorophyllin can produce complexes with Trp-P-2 and Trp-P-2(NHOH). A straightforward mechanism of these inhibitions is that Trp-P-2 [and Trp-P-2(NHOH)] becomes no longer available to organisms on forming the chlorophyll complex.Keywords
This publication has 13 references indexed in Scilit:
- Antimutagenic activity of some naturally occurring compounds towards cigarette-smoke condensate and benzo[a]pyrene in the Salmonella/microsome assayMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1985
- MUTAGENIC POTENCY OF HETEROCYCLIC AMINES IN THE DROSOPHILA WING SPOT-TEST AND ITS CORRELATION TO CARCINOGENIC POTENCY1985
- Somatic mutation and recombination test in Drosophila melanogasterEnvironmental Mutagenesis, 1984
- Syntheses of hydroxyamino, nitroso and nitro derivatives of Trp-P-2 and Glu-P-1, amino acid pyrolysate mutagens, and their direct mutagenicities towards Salmonella typhimurium TA98 and TA98NRCarcinogenesis: Integrative Cancer Research, 1983
- Inhibitory effect of hemin on the mutagenic activities of carcinogensCancer Letters, 1980
- Isolation and characterization of active metabolites of tryptophan-pyrolysate mutagen, TRP-P-2, formed by rat liver microsomesChemico-Biological Interactions, 1980
- Detection of potent mutagens, Trp-P-1 and Trp-P-2, in broiled fishCancer Letters, 1980
- Antimutagenic activities of common vegetables and their chlorophyll contentMutation Research/Genetic Toxicology, 1980
- Inhibition of the mutagenicity of amino acid pyrolysis products by hemin and other biological pyrrole pigmentsBiochemical and Biophysical Research Communications, 1980
- Mutagenicities of N-nitrosamines on salmonellaMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1977