Deacylation of myelin proteolipid protein in organic solvents
- 1 January 1985
- journal article
- research article
- Published by Wiley in Journal of Neuroscience Research
- Vol. 14 (2) , 197-205
- https://doi.org/10.1002/jnr.490140205
Abstract
A procedure has been developed for the deacylation of the hydrophobic, myelin proteolipid apoprotein using hydroxylamine in an alkaline organic solvent medium. Complete removal of covalently bound fatty acids was obtained after 4 hr of treatment. After deacylation, no changes could be detected in the electrophoretic pattern or in the number of free sulfhydryl groups. The deacylated apoprotein remains soluble in chloroform-methanol mixtures and is suitable for further physicochemical characterization.Keywords
This publication has 24 references indexed in Scilit:
- Covalent lipoprotein from the outer membrane of escherichia coliPublished by Elsevier ,2003
- Amino acid sequence of bovine white matter proteolipidArchives of Biochemistry and Biophysics, 1983
- Fatty acid acylation of eucaryotic cell membrane proteinsBiochimica et Biophysica Acta (BBA) - Reviews on Biomembranes, 1982
- Rapid purification of proteolipids from rat brain subcellular fractions by chromatography on a lipophilic dextran gelJournal of Chromatography B: Biomedical Sciences and Applications, 1982
- Myelin lipophilin-induced demyelinating disease of the central nervous systemNeurochemical Research, 1980
- Isoelectric focusing of proteolipidJournal of Neurochemistry, 1978
- Preparation of the proteolipid apoprotein from bovine heart, liver and kidneyBiochimica et Biophysica Acta (BBA) - Protein Structure, 1976
- Isolation of Sarcoplasmic Reticulum ProteinsCold Spring Harbor Symposia on Quantitative Biology, 1973
- Modification of the lowry procedure for the analysis of proteolipid proteinAnalytical Biochemistry, 1972
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970