Somatic hybrid plants obtained by protoplast fusion between Citrus sinensis and Poncirus trifoliata
- 1 November 1985
- journal article
- research article
- Published by Springer Nature in Theoretical and Applied Genetics
- Vol. 71 (1) , 1-4
- https://doi.org/10.1007/bf00278245
Abstract
Somatic hybrid plants of Rutaceae were obtained by protoplast fusion between Citrus sinensis Osb. (‘Trovita’ orange) and Poncirus trifoliata. Protoplasts isolated from embryogenic cells of C. sinensis and from leaves of P. trifoliata, and the culture of fusion products in the presence of high concentrations of sucrose were essential requirements for the selection of hybrids. Green globular embryoids derived from protoplasts resulted in the regeneration of trifoliate plants. Other morphological characters of these plants were intermediate between both parents. The chromosome number in one of the hybrid plants was 36, which was the sum of C. sinensis (2n=18) and P. trifoliata (2n=18). EcoRI restriction analysis of rDNA confirmed the presence of parental nuclear DNAs in the hybrid.Keywords
This publication has 5 references indexed in Scilit:
- Detection of two different nuclear genomes in parasexual hybrids by ribosomal RNA gene analysisTheoretical and Applied Genetics, 1983
- Plant regeneration from "Trovita" orange protoplasts.Ikushugaku zasshi, 1983
- Effect of Carbohydrates on Somatic Embryogenesis in Subcultured Nucellar Callus of Citrus CultivarsZeitschrift für Pflanzenphysiologie, 1982
- Somatic hybridization between male sterile Nicotiana tabacum and N. glutinosa through protoplast fusionTheoretical and Applied Genetics, 1982
- Plant regeneration from Citrus protoplasts: Variability in methodological requirements among cultivars and speciesTheoretical and Applied Genetics, 1982