SPECTROPHOTOMETRIC DETERMINATION OF URINARY OXALATE WITH OXALATE OXIDASE PREPARED FROM MOSS

  • 1 January 1980
    • journal article
    • research article
    • Vol. 26  (7) , 827-830
Abstract
A novel spectrophotometric enzymic procedure for estimating oxalic acid in urine is described. Oxalate oxidase, prepared from moss species (Mnium menziesii, M. insigne, M. ovale, Hylocomium splendens, Eurhynchium stokesii and Rhytidiadelphus triquetrus), converts oxalic acid to H2O2 and CO2. H2O2 is determined enzymatically with horseradish peroxidase, by oxidative coupling of 3-methyl-2-benzothiazolinone hydrazone with N,N-dimethylaniline; the resulting indamine dye is determined spectrophotometrically at 595 nm. Interfering substances are removed by adsorption to ion-exchange resins and oxidation with charcoal, thus avoiding oxalate recovery problems accompanying oxalate isolation. The procedure is rapid, sensitive, linear and precise. Results agreed well with those obtained with a widely used chemical technique.

This publication has 4 references indexed in Scilit: