Response of marine bacterioplankton to differential filtration and confinement

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Abstract
The bacterioplankton community of confined seawater at 25.degree. C changed significantly within 16 h of collection. Confinement increased colony-forming units, total cell number (by epifluorescence microscopy) and average cell volume of bacterioplankton and increased the turnover rate of amino acids in seawater sampled at Frying Pan Shoals, North Carolina, USA. The bacterioplankton community was characterized by 2 components: differential doubling times during confinement shifted dominance from bacteria which were nonculturable to bacteria which were culturable on a complex nutrient medium. Culturable cells (especially those of the genera Pseudomonas, Alcaligenes and Acinetobacter) increased from 0.08% of the total cell number in the seawater immediately after collection to 13% at 16 h and 41% at 32 h of confinement. Differential filtration before confinement indicated that particles passing through a 3.0 .mu.m, but retained by a 0.2 .mu.m, pore-size Nuclepore filter may be a major source of primary amines to the confined population. The 3.0 .mu.m filtration increased growth rate and ultimate numbers of culturable cells through the removal of bacterial predators or the release of primary amines from cells damaged during filtration or both.