A Technic for Determination of Adrenocorticotrophin in Blood.
- 1 March 1952
- journal article
- research article
- Published by Frontiers Media SA in Experimental Biology and Medicine
- Vol. 79 (3) , 432-436
- https://doi.org/10.3181/00379727-79-19403
Abstract
The oxycellulose technic for isolation of ACTH from pituitary has been successfully employed to concentrate and quantitatively to measure blood ACTH. The method (1) appears to be specific for ACTH since hypophysectomized rat blood induces no adrenal ascorbic acid depletion in the assay animal, (2) almost completely recovers ACTH added to hypophysectomized rat blood, (3) almost completely recovers endogenous ACTH, (4) yields concentrates which are not toxic to the assay animal and (5) is reproducible. It is estimated that rat blood, obtained by acute exsanguination of intact animals, contains 2 to 3 m[mu] ACTH per 100 ml.,whereas blood obtained under similar conditions from rats bilaterally adrenalectomized for one week contains 10 to 12 m[mu] ACTH/ 100 ml.Keywords
This publication has 1 reference indexed in Scilit:
- INCREASE IN THE FORMATION AND SECRETION OF ACTH FOLLOWING ADRENALECTOMY1Endocrinology, 1951