INTERACTION OF SOLUBLE RIBONUCLEIC ACID AND MICROSOMES

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Abstract
By using P32-, C14-amino acid-labeled S-RNA it has been possible to study the simultaneous incorporation of S-RNA into microsomal RNA and of C14-amino acid into microsomal protein. The kinetics are consistent with the concept that the admission of an amino acid into peptide linkage requires a large fraction of the associated S-RNA molecule to become firmly and transitorily bound to microsomal RNA. This is further substantiated by experiments in which microsomes previously equilibrated with P32-S-RNA are shown to release the latter on further incubation with unlabeled S-RNA and cofactors necessary for protein synthesis. Evidence that there is no detectable fragmentation of S-RNA to smaller utilizable pieces is also presented.