Darstellung von Thrombozytenmembranproteinen mit monoklonalen Antikörpern im durchflußzytometrischen Bio-Assay

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Abstract
Formation of a hemostatic plug is trigered by platelets. Platelet function (e.g. adhesion, aggregation) depends essentially on membrane bound receptorproteins. Conventional chromatografic analysis of these glycoprotein macromolecules is difficult and not appropriate for diagnostic routine. In combination of cytoflowmetric single cell analysis with monoclonal staining we developed a bio-assay for qualitative and semi-quantitative analysis of glycoprotein IB and IIB/IIIA on vital fixed platelets. The expression of these molecules was evaluated in 20 healthy volunteers. The assay offers for the first time the possibility of screening the expression of receptor proteins on platelet membranes, which are related to indicate either a functional lack in bleeding disorders or a prethrombotic state due to an enhanced functional potential in high risk patients.