Genetic polymorphism of λ1 and λ3 immunoglobulin light chains in theMus subgenus

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Abstract
Several inbred or partially inbred mouse strains derived from wild mice of different Mus subgroups were surveyed for expression of λ1 light chain. One strain, SPE, expressed little or no λ1 chains. Two populations of antibodies were obtained by immunization of SPE mice with λ1 light chain isolated from the BALB/c J558 (α,λ1) myeloma protein. One population of antibodies recognized λ1 allotypic determinants located on the C terminal fragment beginning at the residue 176. A second population was directed against epitopes present on both λ1 and λ3 chains. These determinants, which were detected in all strains tested with the exception of SPE, were located in the V region. This result is in concordance with recent DNA studies showing that the λ1 and λ3 isotype use the same Vλ1 genes (Blomberg, B. et al., Proc. Natl. Acad. Sci. USA 1981. 78: 3765). Whether the V epitopes recognized by SPE antibodies are allotypic or isotypic in nature is not certain.

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