Biochemical and immunological characterization of major surface antigens ofSarcocystis muris andS. suicanis cyst merozoites

Publisher Website
Google Scholar
Abstract
Surface proteins ofSarcocystis cyst merozoites were labeled by biotinylation or radioiodination and identified on Western blots after sodium dodecyl sulfatepolyacrylamide gel electrophoresis (SDS-PAGE). The major labeled proteins ofS. muris andS. suicanis have relative molecular masses of 31 and 33 kDa, respectively. Immunoblots performed with the 31-kDa protein and sera of experimentally infected mice or with monoclonal antibodies toS. muris revealed that this protein is immunogenic. Indirect fluorescent antibody tests (IFAT) executed usingSarcocystis cyst merozoites and polyclonal monospecific antibodies obtained from rabbits immunized with homogeneous major surface antigens gave additional evidence for the localization of the identified antigens in the pellicle. Analysis ofS. muris andS. suicanis proteins by two-dimensional gel electrophoresis revealed multiple isoelectric forms.