Studies on vitamin D and related compounds VI: The synthesis and properties of dihydrotachysterol3

Abstract
Whereas dihydrovitamin D3 I and dihydrovitamin D3 II can be isolated from the mixtures obtained on reduction of cholecalciferol with sodium and alcohols, attempts to obtain dihydrotachysterol3 in a crystalline state from such mixtures were unsuccessful. Pure dihydrotachysterol3, however, could be obtained by reducing tachysterol3 with an excess of lithium in liquid ammonia, concentrating by chromatography on alumina, esterification with phenylazobenzoyl chloride and saponification of the ester. Ultra‐violet and infra‐red absorption spectra are given. The capacity of dihydrotachysterol3 to raise the calcium level of the blood (plasma) proved to be about twice as large as that of dihydrotachysterol2.

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