Sulphasalazine inhibition of human granulocyte activation by inhibition of second messenger compounds.
Open Access
- 1 November 1992
- journal article
- research article
- Published by Elsevier in Annals of the Rheumatic Diseases
- Vol. 51 (11) , 1230-1236
- https://doi.org/10.1136/ard.51.11.1230
Abstract
The effects of sulphasalazine on the production of second messenger compounds in human granulocytes have been characterised by various stimuli. The increases in cytosolic calcium, inositol trisphosphate, diacylglycerol, and phosphatidic acid (all important mediators of intracellular signal transduction) triggered by stimulation were inhibited by sulphasalazine. The metabolites 5-amino-salicylic acid and sulphapyridine were less potent inhibitors than the mother compound. It is concluded that sulphasalazine inhibits the synthesis of phosphoinositide derived second messenger compounds at the level of phospholipase C or its regulatory guanosine 5'-triphosphate (GTP) binding protein. Inhibition of phosphatidic acid synthesis was either due to the same mechanism, or to interaction with a phospholipase D regulating GTP binding protein.Keywords
This publication has 34 references indexed in Scilit:
- Suppressive effect of anti-rheumatic drugs on interleukin-1β release from human peripheral blood monocytesInternational Journal of Immunopharmacology, 1991
- Fluoride-mediated activation of the respiratory burst in electropermeabilized neutrophilsBiochimica et Biophysica Acta (BBA) - Molecular Cell Research, 1990
- Effect of sulphasalazine and sulphapyridine on neutrophil superoxide production: role of cytosolic free calcium.Annals of the Rheumatic Diseases, 1990
- Inhibition of human neutrophil activation by the allergic mediator release inhibitor, CI-949: Mechanism of inhibitory activityBiochemical and Biophysical Research Communications, 1990
- ATP stimulates secretion in human neutrophils and HL60 cells via a pertussis toxin‐sensitive guanine nucleotide‐binding protein coupled to phospholipase CFEBS Letters, 1989
- Characterization of the membrane-associated GTPase activity: Effects of chemotactic factors and toxinsJournal of Cellular Biochemistry, 1989
- G protein regulation of receptor signallingImmunology Today, 1988
- Differing calcium requirements for regulatory effects of ATP, ATPγS and adenosine on O2• responses of human neutrophilsBiochemical and Biophysical Research Communications, 1988
- Studies on the mechanism of inhibition of chemotactic tripeptide stimulated human neutrophil polymorphonuclear leucocyte superoxide production by chloroquine and hydroxychloroquine.Annals of the Rheumatic Diseases, 1987
- The Effect of Sulfasalazine and Its Active Components on Human Polymorphonuclear Leukocyte Function in Relation to Ulcerative ColitisActa Medica Scandinavica, 1979