Crystalline Vitamin B 12

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Abstract
Purification of clinically active liver fractions has led to the isolation, in minute amts., of a crystalline compd., vit. B12, which is highly active for the growth of Lactobacillus lactis. Its potency is about 11 million LLD units/mg., and 0.000013 [gamma]/ml. of culture medium is capable of supporting half-maximal growth under the conditions used. The compd. crystallizes as small red needles. The biologic activity is very high in terms of its activity in tests on pernicious anemia. Clinical response should be obtained from the parenteral administration of 1 [gamma]/day. It is customary to give 20-60 U.S.P. units of liver extract during the first 2-3 days to start remission of pernicious anemia in relapse. This dose range is equivalent to not more than 20-60 [gamma] of vit. B12.
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