Activity of Vitamin B 12 for the Growth of Lactobacillus lactis

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Abstract
A crystalline compound, vit. B12, has been isolated from liver and is very active hem-atopoietically in pernicious anemia. It has been assayed for LLD activity, and has been found either wholly or partially responsible for the LLD growth activity observed for liver extracts. The minute amt. of the compound needed for growth places the vitamin among the most potent mi-crobiologically active compounds. The liver concentrate standard is free from the other factor, TJ, needed by L. lactis, at levels as high as 500 y/tube. Addition of the liver concentrate, as a source of LLD factor, does not affect the growth of L. casei, L. fermenti, L. arabinosus, Streptococcus faecalis R, and Escherichia coli. LLD factor activity occurs in fairly high amts., in decreasing order, in a papain digest of acid ppt. from cow manure, the acid ppt. from cow manure, fish meal, pancreatin, papain, egg white and yolk, and in lower amts. in alcoholic extract of whey, soybean oil meal, gelatin, zein, and Mylase P. enzyme. The TJ factor is also found, in decreasing order, in the papain digest of acid ppt. from cow manure, the acid ppt. of cow manure, egg yolk, papain, and pancreatin, but in much lower amts. in fish meal, alcoholic extract of whey, soybean oil meal, crude casein, egg white, zein, Mylase P. enzyme, gelatin, and KMnO4-oxidized alcoholic extract of whey. The distr. of the LLD and TJ factor activities in these materials suggests that they may be involved in chicken nutrition.