Mapping of transcription sites of simian virus 40-specific late 16S and 19S mRNA by electron microscopy.
- 1 February 1977
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 74 (2) , 496-500
- https://doi.org/10.1073/pnas.74.2.496
Abstract
Simian virus 40 (SV40) late 19S and 16S mRNAs were annealed to complementary regions of partially melted viral double-stranded SV40(LHpa II) DNA or SV40(LBam HI) DNA. The RNA-DNA hybrid regions within the DNA molecules were visualized as loops [with SV40(LBam HI) DNA] in the electron microscope. The data confirm the previous localizations of the 3' and 5' ends of 16S SV40 mRNA and of the 3' end of late 19S SV40 mRNA. The 5' end of the major stable SV40 late 19S mRNA has been positioned at 0.755 map unit. Thus, the sequences of viral DNA from 0.655 to 0.755 map unit, including the replication origin, are not converted into major stable species of late viral mRNA.Keywords
This publication has 28 references indexed in Scilit:
- Mapping of adenovirus messenger RNA by electron microscopy.Proceedings of the National Academy of Sciences, 1976
- Construction and analysis of viable deletion mutants of simian virus 40Journal of Virology, 1976
- Mapping of the three species of polyoma mRNA.Proceedings of the National Academy of Sciences, 1976
- Integrated simian virus 40 sequences in transformed cell DNA: analysis using restriction endonucleases.Proceedings of the National Academy of Sciences, 1976
- Topography of polyoma virus messenger RNA moleculesCell, 1976
- Isolation and characterization of poly(A)-containing polyoma "early" and "late" messenger RNAs.Nucleic Acids Research, 1976
- Mapping the transcription site of the SV40-specific late 16 S mRNANucleic Acids Research, 1975
- A map of temperature-sensitive mutants of simian virus 40Virology, 1975
- Metabolism of the polyadenylate sequence of nuclear RNA and messenger RNA in mammalian cellsCell, 1975
- The Cell-free Translation of SV40 Messenger RNACold Spring Harbor Symposia on Quantitative Biology, 1974