Transglycosylic Reactions of Nucleotides of Deoxy Sugars

Abstract
The biosynthesis of .alpha., .alpha.''-trehalose was investigated in a reaction catalyzed with yeast [Saccharomyces cerevisiae] UDP-Glc:glucose-6-phosphate 1-glucosyl transferase [EC 2.4.1.1.5]. The hydroxyls at positions C-2, C-3, C-4 and C-6 were not essential for preserving its substrate properties in this reaction. The affinity of the enzyme and the rate of hexose incorporation into trehalose decreased in following sequence: UDP-glucose, UDP-2-deoxy-D-glucose, UDP-6-deoxy-D-glucose, UDP-4-deoxy-D-glucose and UDP-3-deoxy-D-glucose.

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