Analysis of the black‐eyed pea trypsin and chymotrypsin inhibitor‐α‐chymotrypsin complex
- 9 June 1997
- journal article
- Published by Wiley in FEBS Letters
- Vol. 409 (2) , 121-127
- https://doi.org/10.1016/s0014-5793(97)00419-5
Abstract
The black‐eyed pea trypsin and chymotrypsin inhibitor (BTCI) is a member of the Bowman‐Birk protease inhibitor (BBI) family. The three‐dimensional model of the BTCI‐chymotrypsin complex was built based on the homology to Bowman‐Birk inhibitors with known structures. An extensive theoretical and experimental study of these known structures has been performed. The model confirms the ideas about Bowman‐Birk inhibitor structure‐function relations and agrees well with our experimental data (circular dichroism, IR and light scattering). The electrostatic potentials at the enzyme‐inhibitor contact surface reveal a pattern of complementary electrostatic potentials from which mutations can be inferred that could give these inhibitors an altered specificity.Keywords
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