Application of immunoperoxidase method to electron microscopic observation of plasma protein on polymer surface

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Abstract
The immunoperoxidase technique was applied to observe the electron microscopic presence of human plasma protein on a polymer surface. Two types of polymer material, i.e., Dacron and a newly synthesized heparinized hydrophilic polymer (H‐RSD, Toray Industries, Inc.), showed different reactions to human plasma proteins. The hydrophobic Dacron showed a decreased affinity for plasma albumin, but a greater affinity for fibrinogen, α1‐lipo‐protein, and β‐lipoprotein when compared to H‐RSD. The thrombo‐resistant H‐RSD showed a relatively strong affinity for albumin, but in a spotty or focal manner. Most of the H‐RSD surface did not adsorb albumin. Fibrinogen adsorption was observed in areas smaller than albumin adsorption. The adsorption of fibrinogen appeared to occur through certain material. The α‐lipoprotein and β‐lipoprotein were not adsorbed on the H‐RSD. The immunoperoxidase method is a sensitive and useful technique in studying the interactions between plasma proteins and the surface of polymer materials.