Growth pattern and citrate production in organ cultures of adult rat ventral prostate

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Abstract
Prostate rudiments from Wistar rats were cultured in MEM supplemented with 15% FBS and insulin (2 μg/ml). In situ prostate acinar epithelium consists of tall columnar and low basal cells. Ultrastructure of the columnar cells is that of typical secretory cells; however, the basal cells are poor in cell organelle. During the first week of culture many secretory cells degenerated and were replaced by cuboidal or low columnar cells. In the presence of testosterone (2 μg/ml) the secretory cells remained viable for 8–10 days before undergoing necrosis. At the termination of the experiment the acinar epithelium consisted of low cuboidal cells. The cultures showed a pattern of citrate production which reached a maximum at 5 days and remained relatively constant thereafter. Ultrastructure of 1‐week cultures exhibited Golgi complexes with dilated cisternae. Although a paucity of cell organelle was found in 7‐day cultures, older cultures (19 days) exhibited morphologic characteristics of normal secretory cells.