FACTORS INFLUENCING VARIOLA VIRUS GROWTH ON THE CHORIOALLANTOIC MEMBRANE OF EMBRYONATED EGGS

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Abstract
A latent period which varied inversely with the strength of inoculum preceded the growth of variola virus which attained a peak growth level in 48 hours regardless of viral dose. The rate of maximum viral growth, 103.8-fold/10 hours, was independent of the virus quantity inoculated. A 35[degree] C incubation temperature was more favorable for viral growth than 37 or 39[degree] C. Varying the dose of inoculum markedly influenced the viral content of membranes, however, no apparent difference was noted when the volume of inoculum was changed. Optimal virus growth occurred in embryonated eggs inoculated at 9 to 13 days of age. Dissemination of virus was greatest throughout the embryonated egg after chorioallantoic membrane inoculation. Limited viral growth and distribution occurred after amniotic, yolk sac, and allantoic injection.