Modulation of Cav3.2 T‐type Ca2+ channels by protein kinase C
- 19 June 2003
- journal article
- Published by Wiley in FEBS Letters
- Vol. 547 (1-3) , 37-42
- https://doi.org/10.1016/s0014-5793(03)00665-3
Abstract
Although T‐type Ca2+ channels have been implicated in numerous physiological functions, their regulations by protein kinases have been obscured by conflicting reports. We investigated the effects of protein kinase C (PKC) on Cav3.2 T‐type channels reconstituted in Xenopus oocytes. Phorbol‐12‐myristate‐13‐acetate (PMA) strongly enhanced the amplitude of Cav3.2 channel currents (∼3‐fold). The augmentation effects were not mimicked by 4α‐PMA, an inactive stereoisomer of PMA, and abolished by preincubation with PKC inhibitors. Our findings suggest that PMA upregulates Cav3.2 channel activity via activation of oocyte PKC.Keywords
This publication has 32 references indexed in Scilit:
- Regulation of the ABC kinases by phosphorylation: protein kinase C as a paradigmBiochemical Journal, 2003
- A Novel Long N-terminal Isoform of Human L-type Ca2+Channel Is Up-regulated by Protein Kinase CJournal of Biological Chemistry, 2002
- Stimulation of recombinant Cav3.2, T‐type, Ca2+ channel currents by CaMKIIγCThe Journal of Physiology, 2002
- Functional Expression and Characterization of a Voltage-Gated CaV1.3 ( 1D) Calcium Channel Subunit from an Insulin-Secreting Cell LineMolecular Endocrinology, 2001
- Cross-talk between G-protein and Protein Kinase C Modulation of N-type Calcium Channels Is Dependent on the G-protein β Subunit IsoformJournal of Biological Chemistry, 2000
- Expression of T-type Ca2+Channels in Ventricular Cells from Hypertrophied Rat HeartsJournal of Molecular and Cellular Cardiology, 1999
- Low-Threshold Calcium Currents in Central Nervous System NeuronsAnnual Review of Physiology, 1996
- Molecular Basis for CA2+ Channel DiversityAnnual Review of Neuroscience, 1994
- Modulation of cardiac Ca2+ channels in Xenopus oocytes by protein kinase CFEBS Letters, 1992
- Activation of protein kinase C by phorbol ester induces downregulation of the Na+/K+-ATPase in oocytes ofXenopus laevisThe Journal of Membrane Biology, 1990