CORTICOSTEROIDS AND THE FUNCTIONAL CAPACITY OF THE RAT HEART-LUNG PREPARATION1,2

Abstract
A technic has been developed for the preparation and maintenance of the isolated rat heart-lung. Index of left ventricular work expressed as cardiac output times mean arterial blood pressure was highly reproducible for a given set of experimental conditions. Order of work performance arranged from highest to lowest was a follows: (1) heart-lung from intact rat perfused with blood from intact rats, (2) heart-lung from adrenalectomized rat perfused with blood from intact rats, (3) intact-adrenalectomized, (4) adrenalectomized-adrenal-ectomized. Corticosterone, but not Substance S, added to the perfusion blood of the intact-adrenalectomized preparation restored work capacity toward that of the intact-intact preparation.

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