CHARACTERIZATION AND USE OF AN ALLOTYPE-SPECIFIC MONOCLONAL-ANTIBODY TO PLACENTAL ALKALINE-PHOSPHATASE IN THE STUDY OF CANCER-RELATED PHOSPHATASE POLYMORPHISM

  • 1 January 1982
    • journal article
    • research article
    • Vol. 42  (6) , 2444-2449
Abstract
The hybridoma technique was used to produce an allotype-specific monoclonal antibody (F11) that reacts with the products of the S, I and D alleles of PLAP [placental alkaline phosphatase] but not of the F allele. Serum and ascites samples from patients with different cancers containing high levels of PLAP were tested for reactivity with F11. These tumor-derived PLAP were of the Nagao type as shown by their sensitivity to inhibition by L-leucine. This type of inhibition is also exhibited by the rare D allelic variant of PLAP but not by the common forms. The Nagao enzyme probably represents reexpression of the D allele of PLAP. F11 reactive and nonreactive samples and samples with intermediate reactivity were found among the cancer sera and ascites. The tumor-derived Nagao enzyme does not represent the reexpression of the D allele but instead, in spite of its distinct inhibition pattern, expresses the same genetic polymorphism that is found in the placenta.

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