Growth inhibition by overexpression of human DEAD box protein rck/p54 in cells of a guinea pig cell line
Open Access
- 16 June 1998
- journal article
- Published by Wiley in FEBS Letters
- Vol. 429 (3) , 279-283
- https://doi.org/10.1016/s0014-5793(98)00605-x
Abstract
We transfected cells of a guinea pig cell line with RCK cDNA inserted in a pIRES1neo expression vector. The overexpression of rck/p54 was confirmed by Western blot and RT‐PCR analysis. In two clones expressing rck/p54, the cell growth was highly inhibited; and their anchorage‐independent growth, which is an important character of malignant transformation, was not found. These findings are the first evidence that the overexpression of a DEAD box protein/RNA helicase could inhibit substantially cell growth at the translational level.Keywords
This publication has 16 references indexed in Scilit:
- Requirement of the DEAD-Box Protein Ded1p for Messenger RNA TranslationScience, 1997
- Xp54, the Xenopus Homologue of Human RNA Helicase p54, is an Integral Component of Stored mRNP Particles in OocytesNucleic Acids Research, 1997
- Identification and chromosome mapping of the mouse homologue of the human gene (DDX6) that encodes a putative RNA helicase of the DEAD box protein familyCytogenetic and Genome Research, 1996
- Chromosomal translocations in human cancerNature, 1994
- Theste13 + gene encoding a putative RNA helicase is essential for nitrogen starvation-induced G1 arrest and initiation of sexual development in the fission yeastSchizosaccharomyces pombeMolecular Genetics and Genomics, 1994
- Efficient Gene Transfer with Less Cytotoxicity by Means of Cationic Multilamellar LiposomesBiochemical and Biophysical Research Communications, 1993
- Induction of apoptosis in fibroblasts by c-myc proteinCell, 1992
- The novel mechanism of initiation of picornavirus RNA translationTrends in Biochemical Sciences, 1990
- The product of the Drosophila gene vasa is very similar to eukaryotic initiation factor-4ANature, 1988
- Cellular tumorigenicity in nude mice: Correlation with cell growth in semi-solid mediumCell, 1974