Auxin Regulation of Flower Bud Formation in Tobacco Explants

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Abstract
Smulders, M. J. M, Janssen, G. F. K, Croes, A. F., Barendse, G. W. M., and Wullems, G. J. 1988. Auxin regulation of flower bud formation in tobacco explants.—J. exp. Bot. 39: 451–459. The auxin 1-naphthaleneacetic acid (NAA) induces flower bud formation in tobacco explants at concentrations between 0.1 and 10 mmol m−3. This regeneration process is completed in 14 d. The presence of NAA in the medium is required only during the first 4 d of culture. In this period the explants take up NAA at a very high rate, which is proportional to the concentration in the medium. Due to the high uptake from a limited volume, the external concentration diminishes and, as a consequence, the uptake rate declines rapidly. A negative correlation was found between medium volume and the NAA concentration optimal for bud formation. It is concluded that it is not the concentration but the dose of hormone taken up during the induction period which determines the number of flower buds formed.